Synthesis of Galactosyl Mannitol Derivative Using β-Galactosidase from Kluyveromyces lactis
نویسندگان
چکیده
منابع مشابه
Synthesis of the Galactosyl Derivative of Gluconic Acid With the Transglycosylation Activity of β-Galactosidase.
Bionic acids are bioactive compounds demonstrating numerous interesting properties. They are widely produced by chemical or enzymatic oxidation of disaccharides. This paper focuses on the galactosyl derivative of gluconic acid as a result of a new method of bionic acid synthesis which utilises the transglycosylation properties of β-galactosidase and introduces lactose as a substrate. Products o...
متن کاملOptimization of β-galactosidase production using Kluyveromyces lactis NRRL Y-8279 by response surface methodology
This paper investigates the production and optimization of β-galactosidase enzyme using synthetic medium by Kluyveromyces lactis NRRL Y-8279 in shake flask cultures. Among the different cell disintegration methods used, the highest specific activity was obtained when the cells were permeabilized using isoamyl alcohol. Response surface methodology was used to investigate the effects of four ferm...
متن کاملApplication of Transgalactosylation Activity of β-Galactosidase from Kluyveromyces lactis for the Synthesis of Ascorbic Acid Galactoside
In view of a commonly known beneficial role and low stability of ascorbic acid, many efforts are constantly undertaken to produce its improved derivatives. This paper presents results on the synthesis of ascorbic acid galactoside using transgalactosylation properties of β-galactosidase from Kluyveromyces lactis and lactose as a donor of galactosyl moiety. The purpose of this study was to determ...
متن کاملSecretion and properties of a hybrid Kluyveromyces lactis-Aspergillus niger β-galactosidase
BACKGROUND The beta-galactosidase from Kluyveromyces lactis is a protein of outstanding biotechnological interest in the food industry and milk whey reutilization. However, due to its intracellular nature, its industrial production is limited by the high cost associated to extraction and downstream processing. The yeast-system is an attractive method for producing many heterologous proteins. Th...
متن کاملEffect of Glutaraldehyde on the Conformational Properties of Kluyveromyces lactis β-Galactosidase and Its Application to Sucrose Biosensor Preparation
The effect of glutaraldehyde on the conformational properties of Kluyveromyces lactis β-galactosidase was determined. The optimum substrate of the enzyme interchanged from lactose to sucrose when it reacted with a certain concentration of glutaraldehyde. Its mechanism was studied by detecting the changes in fluorescence absorption. This is the first discovery that β-galactosidase can be convert...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Polish Journal of Food and Nutrition Sciences
سال: 2017
ISSN: 1230-0322
DOI: 10.1515/pjfns-2016-0002